Effect of filter on specific [3H]-5-hydroxytryptamine binding to synaptic membranes.
نویسندگان
چکیده
In the present study, the authors carried out some basic examinations of specific ( 3HJ-5-Hydroxytryptamine (5-HT, serotonin) receptor binding assay. The most adequate incubation condition for ( 3HJ-5-HT binding assay was at 0°C for 10 to 30 min. Scatchard plot of ( 3HJ-5-HT binding using a Millpore filter and a Glass filter drew biphasic and · monophasic, respectivly. It is suggested that filter may affect on specific ( 3HJ-5-HT binding. Therefore, the characteristics of filter should be taken into consideration in ( 3HJ-5-HT binding assay.
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عنوان ژورنال:
- Hiroshima journal of medical sciences
دوره 31 2 شماره
صفحات -
تاریخ انتشار 1982